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Aminov RF, Aminova AS, Makyeyeva LV, Frolov OK, Fedotov ER, Piatyhorets TV. The effect of water-salt extract of the medicinal leech Hirudo verbana on the regenerative properties of excisional wounds of rat skin. Bìol Tvarin. 2025; 27 (1): 21–26. DOI: 10.15407/animbiol27.01.021.
https://doi.org/10.15407/animbiol27.01.021
Received 29.01.2025 ▪ Revision 14.03.2025 ▪ Accepted 20.03.2025 ▪ Published online 11.04.2025

The effect of water-salt extract of the medicinal leech Hirudo verbana on the regenerative properties of excisional wounds of rat skin

R. F. Aminov, A. S. Aminova, L. V. Makyeyeva, O. K. Frolov, E. R. Fedotov, T. V. Piatyhorets
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Zaporizhzhia National University, Laboratory of Cell and Organismal Biotechnology of the Research Department, 66 Universytetska str., Zaporizhzhia, 69011, Ukraine

The process of skin wound healing begins immediately after injury. Wounds remain one of the most pressing scientific and practical problems of modern veterinary medicine. Treatment of skin wounds in our time requires the use of therapy that promotes wound cleansing and facilitates their healing. In recent years, scientists have focused on the study of natural biologically active substances capable of accelerating wound healing and affecting other diseases. Among them a special place is occupied by medicinal leeches, which have a wide range of therapeutic effects due to the presence of over 100 biologically active substances in their body. An important factor in accelerating the healing of skin wounds is perfusion, such an ability is possessed by leech saliva. Given their significant effects, the study of the effect of water-salt extract of Hirudo verbana on the regenerative properties of excisional wounds has become relevant. The results of the study indicate that the water-salt extract for topical application accelerates wound cleansing and reparative processes. Moreover, on the 14^th day, the wound in animals of the experimental group almost completely healed. On the 30^th day, the wounds in the experimental group of rats completely healed, epithelialized and began to actively be covered with hair.

Key words: excisional wounds, skin, medicinal leeches, Hirudo verbana, biologically active substances, rats

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