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Chechet O, Vishchur O, Kovalenko V, Kurtyak B, Smolyaninov K, Voltornistyi A. The activity of immunological indicators in chickens with the complex use of a symbiotic drug “Biomagn” and a disinfectant “Diolide”. Bìol Tvarin. 2025; 27 (1): 41–47. DOI: 10.15407/animbiol27.01.041.
https://doi.org/10.15407/animbiol27.01.041
Received 03.01.2025 ▪ Revision 18.02.2025 ▪ Accepted 20.03.2025 ▪ Published online 11.04.2025

The activity of immunological indicators in chickens with the complex use of a symbiotic drug “Biomagn” and a disinfectant “Diolide”

O. Chechet¹, O. Vishchur^2,3, V. Kovalenko¹, B. Kurtyak³, K. Smolyaninov², A. Voltornistyi²
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¹State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, 30 Donetska str., Kyiv, 03151, Ukraine
²Institute of Animal Biology NAAS, 38 V. Stus str., Lviv, 79034, Ukraine
³Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, 50 Pekarska str., Lviv, 79010, Ukraine

The goal of the research was to determine the effect of the complex use of symbiotic and disinfectant on immunological indicators in broiler chickens. The research was carried out on ROSS-308 cross broiler chickens. Chickens 1–41 days old (2 groups of 100 chickens) were kept in standard production premises with free access to feed and water, observing the technological parameters of cultivation. The chickens of the research group, in addition to the standard diet, were fed the synbiotic “Biomagn” (0.5 kg/t compound feed) according to the scheme and throughout the experiment they drank the drug “Diolide” with water (1 mg/l). Blood for immunological studies was collected on 10–27–34–41 days. The study was conducted without violating the guidelines of the EU Directive 2010/63/EU on the protection of animals used for scientific purposes. Complex application of the proposed synbiotic and disinfectant to chickens contributed to an increase in the level of immunological reactivity, as indicated by higher (P<0.05–0.01) bactericidal and lysozyme activity of blood serum and phagocytic activity of blood pseudoeosinophils of chickens compared to the control, an optimizing effect on the level of circulating immune complexes. So, the results of the research indicate the additive effect of the synbiotic drug “Biomagn” and the disinfectant “Diolide” on the functioning of the mechanisms of natural protection of broiler chickens during their growing period. This effect is due to the complex normalizing effect of the applied agents.

Key words: broiler chickens, symbiotic, chlorine dioxide, bactericidal, lysozyme and phagocytic activity

1. Adeola AO, Paramo L, Fuoco G, Naccache R. Emerging hazardous chemicals and biological pollutants in Canadian aquatic systems and remediation approaches: A comprehensive status report. Sci Total Environ. 2024; 954: 176267. DOI: 10.1016/scitotenv.2024.176267.
2. Barratt MD, Rodford RA. The computational prediction of toxicity. Curr Opin Chem Biol. 2001; 5 (4): 383–388. DOI: 10.1016/S1367-5931(00)00218-0.
3. Brandariz-Núñez D, Balado-Alonso AM, De La Cámara-Gómez M, Fandiño-Orgueira JM, Martín-Herranz MI. Toxicity induce by chlorine. Farm Hospital. 2022; 46 (5): 308–310. DOI: 10.7399/fh.13221.
4. Bringer MA, Gabrielle PH, Bron AM, Creuzot-Garcher C, Acar N. The gut microbiota in retinal diseases. Exp Eye Res. 2022; 214: 108867. DOI: 10.1016/j.exer.2021.108867.
5. Cash BD. Emerging role of probiotics and antimicrobials in the management of irritable bowel syndrome. Curr Med Res Opin. 2014; 30 (7): 1405–1415. DOI: 10.1185/03007995.2014.908278.
6. Catlin NR, Willson CJ, Creasy DM, Rao DB, Kissling GE, McIntyre BS, Wyde M. Differentiating between testicular toxicity and sexual immaturity in ortho-phthalaldehyde inhalation toxicity studies in rats and mice. Toxicol Pathol. 2018; 46 (7): 753–763. DOI: 1177/0192623318801790.
7. Chechet OM, Kovalenko VL. Immunomodulatory probiotic Biomagn. Technical Conditions TC U 24.2-00699690‒003. 2022: 18 p.
8. Coty JB, Oliveira EE, Vauthier C. Tuning complement activationand pathway through controlled molecular architecture of dextran chains in nanoparticle corona. Intern J Pharmac. 2017; 532 (2): 769–778. DOI: 10.1016/j.ijpharm.2017.04.048.
9. Du Y, Lv XT, Wu QY, Zhang DY, Zhou YT, Peng L, Hu HY. Formation and control of disinfection byproducts and toxicity during reclaimed water chlorination: A review. J Env Sci. 2017; 58: 51–63. DOI: 10.1016/j.jes.2017.01.013.
10. El-Shehawy R, Gorokhova E, Fernández-Piñas F, del Campo FF. Global warming and hepatotoxin production by cyanobacteria: What can we learn from experiments? Water Res. 2012; 46 (5): 1420–1429. DOI: 10.1016/j.watres.2011.11.021.
11. Guarner F, Sanders ME, Szajewska H, Cohen H, Eliakim R, Herrera-deGuise C, Karakan T, Merenstein D, Piscoya A, Ramakrishna B, Salminen S, Melberg J. World gastroenterology organisation global guidelines: Probiotics and prebiotics. J Clin Gastroenterol. 2024; 58 (6): 533–553. DOI: 10.1097/MCG.0000000000002002.
12. Ho CS, Wong CTH, Aung TT, Lakshminarayanan R, Mehta JS, Rauz S, McNally A, Kintses B, Peacock SJ, Fuente-Nunez C, Hancock REW, Ting DSJ. Antimicrobial resistance: a concise update. Lancet Microb. 2025; 6 (1): 100947. DOI: 10.1016/lanmic.2024.07.010.
13. Klaenhammer TR, Kleerebezem M, Kopp MV, Rescigno M. The impact of probiotics and prebiotics on the immune system. Nat Rev. Immunol. 2012; 12 (10): 728–734. DOI: 10.1038/nri3312.
14. Koenen ME, Kramer J, van der Hulst R, Heres L, Jeurissen SHM, Boersma WJA. Immunomodulation by probiotic lactobacilli in layer- and meat-type chickens. British Poult Sci. 2004; 45 (3): 355–366. DOI: 10.1080/00071660410001730851.
15. Kovalenko VL, Ponomarenko GV, Kukhtyn MD, Paliy AP, Bodnar OO, Rebenko HI, Kozytska TG, Makarevich TV, Ponomarenko OV, Palii AP. Evaluation of acute toxicity of the «Orgasept» disinfectant. Ukr J Ecol. 2020; 10 (4): 273–278. DOI: 10.15421/2020_1982.
16. Kucheruk MD, Zasekin DA. The influence of prophylactic biopreparations on preservation and microbiocenosis of chickens. Sci Mess LNUVMBT Ser Vet Sci. 2019; 21 (94): 44–50. DOI: 10.32718/nvlvet9408.
17. Ma JW, Huang BS, Hsu CW, Peng CW, Cheng ML, Kao JY, Way TD, Yin HC, Wang SS. Efficacy and safety evaluation of a chlorine dioxide solution. Intern J Environ Res Publ Health. 2017; 14 (3): 329. DOI: 10.3390/ijerph14030329.
18. Orishchuk OS, Tsap SV. Scientific and practical basis for the use of probiotics to improve the quality of poultry products. Theor Appl Vet Med. 2020; 8 (4): 241–245. DOI: 10.32819/2020.84034.
19. Placha I, Pogany Simonova M, Cobanova K, Laukova A, Faix S. Effect of Enterococcus faecium AL41 and Thymus vulgaris essential oil on small intestine integrity and antioxidative status of laying hens. Res Vet Sci. 2010; 89 (2): 257–261. DOI: 10.1016/j.rvsc.2010.03.006.
20. Prokopenko OO, Smolyaninov KB, Vishchur OI, Mudrak DI, Broda NA, Masyuk MB, Smolyaninova OO, Voltornisty AV. The effect of drugs “Enteronormin” and “Zeleris” on the antioxidant potential of young calves. Bìol Tvarin. 2024; 26 (4): 55–59. DOI: 10.15407/animb04.055.
21. Prokopenko O, Vishchur O, Romanovych M, Levkivska N, Sobko G. The influence of the synbiotic preparation, Iodine and Selenium, on the state of natural and adaptive protec-tion of calves. Abstracts of reports of the Conference “Modern Methods of Diagnosis, Treatment and Prevention in Veterinary Medicine”, Lviv, 2021: 124–125. (in Ukrainian)
22. Razanova O, Yaremchuk O, Gutyj B, Farionik T, Novgorodska N. Dynamics of some mineral elements content in the muscle, bone and liver of quails under the apimin influence. Sci Horizons. 2022; 25 (5): 22–29. DOI: 10.48077/scihor.25(5).2022.22-29.
23. Romanovych MM. The dynamics of humoral protection factors in broilers under the conditions of probiotic preparations application. Sci Mess LNUVMBT Ser Vet Sci. 2018; 20 (83): 264–267. DOI: 10.15421/nvlvet8352.
24. Sanekata T, Fukuda T, Miura T, Morino H, Lee C, Maeda K, Araki K, Otake T, Kawahata T, Shibata T. Evaluation of the antiviral activity of chlorine dioxide and sodium hypochlorite against feline calicivirus, human influenza virus, measles virus, canine distemper virus, human herpesvirus, human adenovirus, canine adenovirus and canine parvovirus. Biocontrol Sci. 2010; 15 (2): 45–49. DOI: 10.4265/bio.15.45.
25. Slivinska LG, Yaremchuk VY, Shcherbatyy AR, Gutyj BV, Zinko HO. Efficacy of hepatoprotectors in prophylaxis of hepatosis of laying hens. Reg Mech Biosys. 2022; 13 (3): 287–293. DOI: 10.15421/022237.
26. Sokoliuk VM, Zasiekin DA. Sanitary and hygienic evaluation of the water on the example of one of the farms of NULES of Ukraine. Vet Biotechnol. 2016; 28: 265–271. Available at: https://vetbiotech.kiev.ua/en/archives/35-28/466-sokoluk-v-m (in Ukrainian)
27. Sorlini S, Gialdini F, Biasibetti M, Collivignarelli C. Influence of drinking water treatments on chlorine dioxide consumption and chlorite/chlorate formation. Water Res. 2014; 54: 44–52. DOI: 10.1016/j.watres.2014.01.038.
28. Tam M, Gómez S, González-Gross M, Marcos A. Possible roles of magnesium on the immune system. Eur J Clin Nutr. 2003; 57 (10): 1193–1197. DOI: 10.1038/sj.ejcn.1601689.
29. Tsilingiri K, Barbosa T, Penna G, Caprioli F, Sonzogni A, Viale G, Rescigno M. Probiotic and postbiotic activity in health and disease: Comparison on a novel polarised ex-vivo organ culture model. 2012; 61 (7): 1007–1015. DOI: 10.1136/gutjnl-2011-300971.
30. Urdaci MC, Bressollier P, Pinchuk I. Bacillus clausii probiotic strains: Antimicrobial and immunomodulatory activities. J Clin Gastroenterol. 2004; 38 (6): 86–90. DOI; 10.1097/01.mcg.0000128925.06662.69.
31. Yu CH, Huang TC, Chung CC, Huang HH, Chen HH. Application of highly purified electrolyzed chlorine dioxide for tilapia fillet disinfection. Sci World J. 2014: 619038. DOI: 10.1155/2014/619038.
32. Zakharenko MO, Polyovyi LV, Poliakovskyi VM, Shevchenko LV, Yaremchuk OS. Sanitary and Hygienic Requirements for Water and Water Supply of Agricultural Enterprises. A tutorial. Vinnytsia, VNAU Publ., 2011. (in Ukrainian)